Docker image with PacBio secondary analysis tools

I’ve been working a lot with PacBio Hifi data lately. After having zero prior experience with PacBio secondary analysis up until about 7 months ago, I’ve implemented a WGS and RNA-seq Nextflow pipeline.

Those pipelines utilize Seqera Wave containers, that are built on the fly as the pipeline runs.

But today I made a public Docker image with the PacBio secondary analysis tools that includes the following tools:

• pbmm2

• hificnv

• pbsv

• hiphase

• trgt

• pb-CpG-tools

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Here is the URI for the Docker image:

public.ecr.aws/q3t0i2q1/indapa-pbtools:latest

Feel free to use it in your Nextflow pipelines or other workflows.